Sara Salehi Hammerstad
Position: Endocrinologist MD. PhD student
Place of work: Dept of Endocrinology, Oslo University Hospital, Aker
Phone: +47 22 89 40 00
Childhood diabetes - Knut Dahl-Jørgensen
Current research focus
Virus, genetics and autoimmunity in thyroiditis. A biopsy study.
Summary of the project:
The aim of this study is to verify whether viruses may cause thyroid dysfunction in genetically predisposed patients. Thyroid biopsies will be performed to study whether a virus infection is present, to isolate the viruses and possibly its receptors.
Detect viruses and virus receptors directly in thyroid tissue in patients newly diagnosed with AITD.
Describe the related immunological processes.
Search for genetic factors, predisposing or modifying AITD.
Methods: Patients with thyroid disease will be recruited in early stage of the disease. In addition thyroid tissue from control patients will be obtained to define normal values.
Design: Prospective controlled study.
Patients: 10-40 in each group. Age 18-50. Control patients: N: 50
Thyroid biopsy procedure has been approved by the Norwegian Government’s Ethics Committee. Biopsies are taken as soon as the diagnosis is established. We plan to perform the following examinations:
Histology, electron microscopy and immunohistochemistry of thyroid tissue.
mRNA extraction and microarray screening analysis for viruses in tissue samples from the thyroid and virus verification by PCR , in situ hybridisation and immunochemistry.
Virus isolation from stool, blood samples, and thyroid biopsies.
Sequencing of viruses found.
Gene expression analysis: “Whole genome” microarray analysis from thyroid tissue.
Comparison with control group samples for identification of candidate susceptibility genes for thyroid disease.